Genetic Diversity of African Catfish (Clarias Gariepinus Burchell, 1822) from Lakes Alau and Maladumba using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SdsPage)
Abstract
The genetic diversity of Clarias gariepinus from Lakes Alau and Maladumba were investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with the aims of improvement, management and conservation of the populations in the Lakes. Blood serum was collected from each of the wild Clarias gariepinus. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was carried out using Bio-Rad Mini Protein II cell, 10 ml capacity. Gel images were analyzed using Gen ALEx (version 6.1) software. The results showed the mean number of alleles among the two strains to be 2.000±0.000. The effective number of alleles from Maladumba and Alau Clarias gariepinus were 1.687±0.033 and 1.689±0.000, respectively. Shannon’s information index for the two strains were 0.583±0.016 and 0.593±0.013, respectively. Both strains were 100% polymorphic. The unbiased expected heterozygosity was 0.402±0.04 for Alau Clarias gariepinus and 0.411±0.012 for Maladumba strain. The mean unbiased expected heterozygosity was found to be 0.407±0.009 for both strains. The expected heterozygosity for Alau and Maladumba were found to be 0.396±0.014 and 0.404±0.012, respectively. The average expected heterozygosity was 0.400±0.009 for both Alau and Maladumba Clarias gariepinus. The genetic distance between the strains was 0.001. While the unbiased genetic identity was 0.999. Similarity coefficient results showed one clade between the two populations. This study revealed higher genetic diversity within population and lower genetic variability between the two strains. This may indicates that the strains are genetically similar.
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Agbebi, O.T., Ajagbe, O., Makinde, L. and Afolabi, O. (2009). Production, growth and effect of varying stocking density of Clariobranchus fry. International Journal of Fisheries, 4(4): 73-78.
Agbebi, O. T., Haboya, D. E. and Adebambo, A. O (2013). Preliminary characterization of genetic strain in Clarrid species, Clarias gariepinus and Heterobranchus bidorsalis. African Journal of Biotechnology, 12 (4): 364369.
Agnese, J. F., Teugels, G. G., Galbusera, P., Guyomard, R. and Volckaert, F. (1997) Morphometric and genetic characterization of sympatric populations of Clarias gariepinus and Clarias anguillaris from Senegal. Journal Fish Biology, 50:1143–1157.
Alam, Md.S., Maraya, J., Hossain, M.Md and Islam, S. (2009). Population genetic structure of three major river populations of Rohu, Labeo rohita (Cyprinidae: Cypriniformes) using microsatellite DNA markers. Genes and genome, 31: 43–51.
Allendorf, F. W, Luikart,. G. H and Aitken, S. N. (2012). Conservation and the genetics of Populations. West Sussex: Wiley-Blackwell, 2: 615-624.
AQUALEX, (2004). Basic techniques in fish haematology. AMC Ltd., http//www.aqualex.org/elearning/fish_haemat ology/english/index.html.
Ardestani, G., Rogelio, D. R. O., Reyes, R. C and Laude, R.P. (2014). Genetic diversity of two Philippine freshwater goby species (Perciformes: Gobiidae): implications for conservation. Aquatic Conservation: Marine Freshwater Ecosystems 24: 1-9; http://dx.doi.org/10.1002/aqc.2462.
Awodiran, M. O and Afolabi, O. (2018). Genetic Diversity in Cultured and Wild Population of Clarias gariepinus (Burchell, 1822) in Nigeria Using Random Amplified Polymorphic DNA (RAPD) and Microsatellite DNA. Fisheries and Aquaculture Journal, 9: 247p.
Barasa, J. E., Abila, R., Grobler, J. P., Dangasuk, O. G., Njahira, M. N and KaundaArara, B. (2014). Genetic diversity and gene flow in Clarias gariepinus from Lakes Victoria and Kanyaboli, Kenya. African Journal of Aquatic Science, 39 (3): 287-293.
Beardmore, A. L, Mair, C. G. and Lewis, C. G. (2008). Biodiversity in aquatic systems in relation to aquaculture. Aquaculture Research, 28: 829–839.
Botstein, D., White, R. L., Skolnick, M. and Davis, R. W. (1980). Construction of genetic linkagemap in man using restriction fragment length polymorphism. American journal of human Genetics, 32:314-331.
Caballero, A and García-Dorado, A. (2013). Allelic diversity and its implications for the rate of adaptation. Article of Genetics 195:1373–1384.
Carlson, S. M., Cunningham, C. J. and Westley, P.A.H. (2015). Evolutionary rescue in a changing world. Trends ecology evolution, 29: 521–530.
Caprette, D. R. (2005). Measuring relative mobility of protein band. Experimental Bioscience. Available on: http://www.ruf.rice.edu/~bioslabs/studies/sds- page/rf.html Accessed on 12/10/2010. 8p.
Chistiakov, D. A., Hallenmans, B. and Volckaert, F. A. M. (2006). Microsatellite and genomic distribution, evolution, function and application: a review with special reference to fish genetics. Aquaculture, 255: 1-29.
Ciftci, Y. and Okumus, O. (2002). Fish Population Genetics and Application of Molecular Markers to Fisheries and Aquaculture – I: Basic Principles of Fish Population Genetics. Turkish Journal Fisheries and Aquatic Sciences, 2: 145-155.
Diyaware, M. Y, Haruna A. B, Abubakar, K. A. and Omitogun, G. O. (2012). Serum protein characterisation of intergeneric hybrids between C. anguillaris and H. bidorsalis and their hybrids from semi-arid zones of Nigeria. Journal of Microbiology and Biotechnology Research, 1(4): 227-234.
Ezilrani, P. and Godwin, C. J. (2015). Genetic variation and differentiation in African catfish, Clarias gariepinus, assessed by heterologous microsatellite DNA. Indian Journal of Biotechnology, 14: 388-393
Fagbenro, O. A., Adedire, C. O., Owoseeni, E. A. and Ayotunde, E. O. (1993). Studies on the biology and aquaculture potential of feral catfish Heterobranchus bidorsalis (Geoffrey St. Hilaire 1809) (Clariidae). Tropical Zoology, 6:67-79.
Foulley, J and Ollivier, L (2006) Estimating allelic richness and its diversity. Article of Livestock Science. 101:150–158.
Garibaldi, L. (1996). List of animal species used in Aquaculture. FAO Fisheries Circular. 91: 173- 177.
George, J., Arumugam, S., Ponnu, E., Radhakrishanan, P. and Ramaiah, S. (2013). Electrophoretic studies on the muscle proteins of three species of genus Puntius (OsteichthyesCyprinidae). International Journal of Research in Fisheries and Aquaculture, 3(2): 16-20.
Ikpeme, E.V., Udensi, O.U., Ekaluo, U.B., Ekooffreh, M.E., Okolo, C.M., Ekpo, P. B. and Ogbonna, N.C. (2015). Unveiling the Genetic Diversity in Clarias geriepinus (Burchell, 1822) Using Random Amplified Polymorphic DNA (RAPD) Fingerling Technique. Asian Journal of Animal Science. 9(5): 187-197.
James, E. B., Sinebongo, M., Romulus, A., Johannes, P. G., Robert, A. S., Hesmari, B., Moses, N. N., Emily, J. C., Otto G. D., Boaz, KaundaArara, L and Erik, V. (2017). Genetic diversity and population structure of the African catfish, Clarias gariepinus (Burchell, 1822) in Kenya: implication for conservation and aquaculture, Belgian Journal of Zoology, 147 (2): 105–127.
Kirpichnikov, V. S. (1992). Adaptive nature of intra populational biochemical polymorphism in fish. Journal of Fish Biology 40(1): 1-16. Lal, K. K., Singh, R. K., Mohindra, V., Singh, B. and Ponniah, A.G. (2003). Genetic Make up of Exotic Catfish Clarias gariepinus in India. Asian Fisheries Science 16: 229-234.
Megbowon, I., Fashina-Bombata, H. A, Akinwale, M. M. A., Hammed, A. M, Okunade, O. A. and Mojekwu, T. O. (2013). Breeding Performance of Clarias gariepinus Obtained From Nigerian Waters. IOSR Journal of Agriculture and Veterinary Science (IOSRJAVS) 6 (3): 6-9. Available on: www.iosrjournals.org
Na-Nakorn, U., Hara, M., Taniguchi, N and Seki, S. (1998). Isozymes Variation of Clarias macrocephalus from Four Locations in Thailand, Fisheries Science, 64(4): 526-530.
Nevo, E. (1984). Genetic variation in natural populations: Patterns and theory. Theoretical Population Biology, 13: 121-177.
Odedoyin, D. O. (2007). Survival and growth of hybrid female Clarias gariepinus and male Heterobranchus longifilis (val) Fingerlings: Effect of broodstock sizes. American-Eurasian Journal of Scientific Research 2(1):19-23.
Park, L. K. and Moran, P. (1995). Developments in molecular genetics techniques in fisheries. G.R. Carvalho and T.J. Pitcher (Eds.), Molecular Genetics in Fisheries. London: Chapman and Hall.
Peakall, R., and Smouse, P. E., (2006). GenALEX 6: Genetic analysis in Excel, Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288295.
Perrier, C., Rene, G., Bagliere, J and Evanno, G. (2011). Determinants of hierarchical genetic structure in Atlantic salmon populations: environmental factors vs. anthropogenic influences. Molecular ecology, 20: 4231– 4245.
Petit, R, Mousadik, A. E. l and Pons, O. (1998). Identifying populations for conservation on the basis of genetic markers. Article of Conservation Biology, 12:844–855.
Popoola, O. M., Fasakin, E. A. and Awopetu, I. J. (2014). Genetic Variability In Cultured and Wild Population of Clarias Gariepinus Using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). British Biotechnology Journal, 4(7): 751-759.
Pujolar, J. M., Maes, G. E., Vancoillie, C. and Volckaert, F. A. M. (2005). Growth rate correlates to individual heterozygosity in European ell, Anguilla anguilla L. Evolution, 59: 645-653.
Saad, Y. M., Hanafi, M.S., Essa, M. A. Guerges, A. A. and Ali,S. F. (2009). Genetic signatures of some Egyptian Clarias gariepinus populations. Global Veterinaria, 3: 503-508.
Soliman, I.. M., Zaghloul, S. M., and Heikal, M. Y. (2014). Genetic variation within and among three Egyptian Mesembryanthemum species using different genetic markers. Egyptian Journal of Basic and Applied Sciences, 1:127-135. Teugels, G. G., Ozouf-Costaz, C., Legendre, M. and Parrent, M. (1992). A karyological analysis of the artificial hybridization between Clarias gariepinus and Heterobranchus longifilis, Pisces: Clariidae. Journal of Fish Biology 40:81-86.
Theophilus, J. and Rao, P. R. (1998). Electrophoretic studies on the serum proteins of the three species of genus Channa, Indian. Indian Journal of Fisheries 35(4): 294-297.
Thorpe, J. P. (1982). The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Annual Review of Ecology and Systematics, 13, 139–168. Thorpe,
J. P. and Sole-Cava, A. M. (1994). The use of allozyme electrophoresis in invertebrate systematics, Zoologica Scripta, 23: 3-18.
Van der Bank, F. H., Grobler J. P. and Du Preez, H. H. (1992). A comparative biochemical genetic study of three populations of domesticated and wild African catfish (Clarias gariepinus). Compendium Biochemistry and Physiology 101B No. 3: 387390.
Wang, Q., Wang X., Xie Z., Li Y., Xiao L., Peng, C., Zhang H., Li S., Zhang, Y and Lin, H. (2016). Microsatellite analysis of the genetic relationships between wild and cultivated giant grouper in the South China Sea. Journal of Genetics, 95, 369-376.
Ward, R. D. and Grewe, M. (1995). Appraisal of molecular genetics techniques in fisheries. G.R. Carvalho and T. J. Pitcher (Eds.), Molecular Genetics in Fisheries. London: Chapman and Hall. Pp. 29-54.
Xia, J. H., Bai, Z., Meng, Z., Zhang, Y., Wang, L., Liu, F., Jing, W., Wan, Z.Y., Li, J., Lin, H and Yue, G. H. (2015). Signatures of selection in tilapia revealed by whole genome resequencing. Science Rep 5, 14168.
Zhao, K., Duan, Z., Peng, Z., Gan, X., Zhang, R., He, S. and Zhao, X. (2011). Phylogeography of the endemic Gymnocypris chilianensis (Cyprinidae): sequential westward colonization followed by allopatric evolution in response to cyclical Pleistocene glaciations on the Tibetan Plateau. Molecular phylogenetic evolution 59: 303–310.
